Part:BBa_J119361:Design

tClone Red: Device for Testing Transciptional Terminators and Riboswitches via Golden Gate Assembly

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1499
  Illegal AgeI site found at 1611
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 849
  Illegal BsaI.rc site found at 42
  

Design Notes

None.

Source

Part J119137 was used to clone the pTac promoter. The resulting plasmid was used in BsmBI GGA to produce J119361.